Nature Biotechnology
A monthly journal covering the science and business of biotechnology, with new concepts in technology/methodology of relevance to the biological, biomedical, agricultural and environmental sciences.
Harnessing the intracellular triacylglycerols for titer improvement of polyketides in Streptomyces
Pharmaceutically important polyketides are produced during the stationary phase of growth of Streptomyces species in fermenters. The source of intracellular metabolites that are funneled into polyketide biosynthesis has proven elusive. We uncover the mystery and devised a titer improvement strategy.
Harnessing Type I CRISPR–Cas systems for genome engineering in human cells
Using a FokI fusion design, we show that Type I CRISPR-Cas systems can be expressed, reconstituted, and faithfully trafficked to the nucleus for genome engineering in human cells