Nature Methods
This journal is a forum for the publication of novel methods and significant improvements to tried-and-tested basic research techniques in the life sciences.
KAS-seq: capturing global transcription activity with kethoxal-assisted labeling
We describe a kethoxal-assisted single-stranded DNA sequencing (KAS-seq) approach. KAS-seq allows rapid (within 5 min), sensitive and genome-wide capture and mapping of ssDNA produced by transcriptionally active RNA polymerases or other processes in situ using as few as 1,000 cells.
Spatial Heterogeneity in Molecular Brightness
Oligomerisation of membrane proteins remains a field characterized by intense research interest, due to the large number of pharmacological and clinical implications that the formation of molecular complexes of signaling proteins carries.
SPARK: finding genes with spatial expression patterns
We present Spatial PAttern Recognition via Kernels (SPARK) as an effective statistical tool to identify genes with spatial expression patterns in spatially resolved transcriptomic studies. A particular feature of SPARK is its ability to produce calibrated p-values for spatial analysis.
