Nature Protocols
This journal publishes secondary research articles and covers new techniques and technologies, as well as established methods, used in all fields of the biological, chemical and clinical sciences.
Pseudotargeted metabolomics: A method for semi-quantitatively monitoring thousand of metabolites
We developed a pseudotargeted metabolomics method, ion-pairs used for MRM are extracted from untargeted mass spectrometry data by using mixtures of samples to be analyzed, semi-quantitative information of each sample is obtained by the MRM without identification of the metabolites.
Multivariate classification for vibrational spectroscopy in biological samples
Vibrational spectroscopy techniques, such as Fourier-transform infrared (FTIR) and Raman spectroscopy, are becoming widely used for biological applications. The use of machine learning algorithms are critical towards extracting important information and visualisation in a readily interpretable form.