A colorful biofilm: detection of three bacterial vaginosis-associated species by PNA-FISH

Published in Microbiology

Share this post

Choose a social network to share with, or copy the shortened URL to share elsewhere

This is a representation of how your post may appear on social media. The actual post will vary between social networks

In our recently published paper on npj Biofilms and Microbiomes, we developed a new multiplex protocol for the detection of three different bacterial species associated with bacterial vaginosis (BV).

BV is a polymicrobial infection, where hundreds of different species can be present, and it is associated with the development of a multispecies biofilm on the vaginal epithelium. Some of the species present in BV seem to have a preponderant role on the development of infection.

Peptide nucleic acid (PNA) probes are widely used for targeting microorganisms in a variety of samples, and coupled with fluorescence in situ hybridization (FISH) analysis it allows the detection of specific species.

In this work, we were able to target three of the most common BV-associated species namely, Gardnerella spp., Fannyhessea vaginae, and Prevotella bivia, and detect them by PNA-FISH.

After the theoretical evaluation of the PNA probes, we performed several in vitro experiments to confirm the sensitivity, specificity, and efficiency of the probes. In an in vitro triple-species biofilm, we could detect the specific species and the result is a colorful biofilm. We could thus observe the structure of the biofilm and the organization of the species within the biofilm (Figure 1).

Figure 1: Confocal laser scanning microscopy images of triple-species biofilms analyzed by PNA-FISH, acquired using two different magnifications (100x and 400x).

Although this new approach can represent possible progression to overcome some of the limitations that the traditional methods for diagnosis of this infection present, it is a great achievement for the observation, analysis, and study of polymicrobial biofilms in BV.

Please sign in or register for FREE

If you are a registered user on Research Communities by Springer Nature, please sign in

Go to the profile of Sudarsan Mugunthan
8 months ago

Very nice work to apply newer version of  FISH to  identify pathogens in a diseased condition.

Follow the Topic

Life Sciences > Biological Sciences > Microbiology

Related Collections

With collections, you can get published faster and increase your visibility.

Live biotherapeutics and medicinal microbiome products

This Collection welcomes contributions on recent developments in the utilization of live biotherapeutics and medicinal microbiome products for the treatment or prevention of diseases. In many cases, suitable drug treatments are still lacking due to the ineffectiveness of existing standards or the presence of long-term side effects. We are particularly interested in difficult-to-treat non-communicable diseases whose pathogenesis is associated with microbiota.

Publishing Model: Open Access

Deadline: Aug 02, 2024