The first few months of Antibodypedia

Published in Protocols & Methods

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This is a guest blog post from Victoria Newman, the Antibodypedia curator.

Late last December I wrote a blog post for Stepwise announcing the launch of the new NPG resource Antibodypedia | nature (, an open-access platform to search for antibody data and commentary. I’ll talk more about what we’re trying to do below; however, as a quick status update, since our launch we’ve been steadily adding antibodies and making improvements here and there and have had lots of positive feedback from users. Most impressively, since the New Year we’ve been accessed from 136 countries, with repeat visitors from 113 countries in the last four weeks. Yay for open-access! But we know there is always room for change and we value your comments. Send us your feedback or, if you’re planning on attending the FASEB Experimental Biology (ASBMB) meeting next week in San Diego, stop by the NPG booth and let me know what you think in person: I’ll be there from 12-3 on the afternoon of Monday the 23rd.

So what does Antibodypedia do? We think of it as a microjournal, publishing bite-sized bits of data rather than full-length manuscripts. The data are specific to assays involving antibodies, since we believe that there’s huge scope for improvement with regard to their annotation—the process of buying them can be intensely chaotic, sometimes with very little information available to suggest that one reagent works better than another in the experiment you’re trying to plan. Our aim is to provide data from the antibody user community (including published references citing reagents) and antibody vendors or distributors to give users as well-rounded as possible a grasp of how a product works for different techniques. Because we feel strongly that your data (the control experiments in your lab notebook that may make it into supplementary data or may never see the light of day again) are the best way to convince other users that reagents do or don’t work, we’ve arranged to assign a DOI to each data submission so they can be cited onward. They don’t have to look lovely, especially if you think they’re evidence that something isn’t working, but we feel that they should be made public to help you troubleshoot your blank western blot. So send us your data—help users to answer the eternal question ‘Did I forget to induce my cultures again?’.

Advanced Search on Antibodypedia

Advanced Search

Because we want to provide researchers with the capacity to identify antibodies proven to work in specific techniques, Antibodypedia is structured slightly differently from many of the antibody search engines you might find out there: its organization is gene-centric, so each antibody directed against a single protein target can be retrieved by a search for that antigen. ‘Advanced search’ lets you find antibodies that are recommended or validated for one or more types of experiment, sold by a particular provider, and linked to reference citations, among other things. The ‘Advanced search’ menu is always undergoing development, so please let us know if it’s missing something you’d find useful.

After you’ve defined and run your search, your results will be displayed as a table, with each row representing a single antibody.

Search Results

Search Results

Clicking on a reagent will take you to antibody pages, which host or link to both user- and provider-sourced data as well as protocols and published references using those reagents.

Antibody Details

Antibody Details

You can also choose some of the antibodies retrieved by your search for side-by-side comparison to decide more easily which one(s) you’d like to test. Our antibody pages link directly out to vendor pages as well so you can find pricing and ordering information.

We think Antibodypedia will simplify selecting an antibody for a particular experiment by making quality control data both accessible to bench scientists and easy to interpret. But in order to maximize our utility, we need your help. Tell us which antibody providers you’d like to see listed in Antibodypedia so we can contact them and which techniques you use that aren’t currently searchable so we can start gathering data for them. And most importantly: submit to us so that we can populate antibody entries with your results! Remember that we’re not asking for publication-quality images, just something that can be interpreted as positive or negative proof that something’s working along with some notes on how you set up your experiment. Help us create an efficient means to search for good antibodies so you and your colleagues can plan your assays with less waste of time and money.

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