The Journey Behind the Research
Abalone, marine gastropods of the genus Haliotis, are ecologically vital, culturally significant, and prized as a premium seafood delicacy. Unfortunately, over 40% of abalone species are currently threatened with extinction. Driven by climate change and overexploitation, both wild and farmed populations are facing alarming declines. Understanding the genetic mechanisms underlying heat stress responses is essential for conserving these remarkable organisms and ensuring the long-term sustainability of abalone aquaculture.
Heat stress is the most widely studied environmental challenge in abalone research, and for good reason. As marine heatwaves become more frequent and intense due to climate change, both wild and farmed abalone populations are increasingly at risk. In response, researchers around the world have been using transcriptomic tools to understand how abalone react at the molecular level when exposed to high temperatures.
Over the years, numerous studies have investigated these responses in various species, under diverse conditions, and at different life stages. However, most of these studies were done independently, each providing only a piece of the puzzle. We realized this growing collection of data represented a unique opportunity: what if we could bring them all together and look for the common genes? In other words, are there core genes or pathways that are consistently activated when abalones face thermal stress, regardless of species, stress intensity or any other experimental factor (other than heat stress)?
To answer this, we conducted a large-scale meta-analysis of publicly available RNA-seq datasets. Our goal was to integrate and reanalyze these studies through a single, consistent pipeline to uncover shared genetic patterns. Ultimately, we hoped to identify the key molecular players that help abalone survive in warming oceans and provide insights that could support climate-resilient aquaculture in the future.
Key Findings
Through rigorous meta-analysis, we identified a remarkable pattern: a core set of 74 genes consistently responded to heat stress across most of the studies. These genes weren’t random as they formed a cohesive network of molecular defenders. What was most exciting, though, was the consistency. Despite differences in geography, species, temperature treatments, and tissue types, the same core genetic response kept showing up. This suggests a conserved heat-response toolkit—a genetic response shaped by years of evolution. The main “tool” (i.e. genes/pathways) identified in this core toolkit included:
- Heat Shock Proteins (HSPs): crucial for protein folding and protection against thermal damage.
- Ubiquitin–Proteasome System (UPS): involved in protein degradation and turnover.
- Alternative Splicing Mechanisms: allowing for versatile gene expression in response to stress.
Implications and Future Directions
Identifying this core genetic response provides a foundation for developing strategies to enhance the resilience of abalone. Potential applications include:
- Selective Breeding: utilizing genetic markers to breed heat-tolerant abalone strains.
- Early Stress Exposure: conditioning juveniles to moderate heat stress to build resilience.
- Gene Editing: exploring targeted interventions to bolster stress-response pathways.
We uncovered a core set of genes, the abalone’s genetic armour, consistently activated under heat stress. These insights unlock pathways for conservation and climate-resilient aquaculture. As marine heatwaves intensify in frequency and severity, findings like these are crucial not only for the conservation of abalone but also for protecting other vulnerable marine species.
BMC Genomics: Second dive into marine genomics