Why Latinas Are More at Risk for Cervical Cancer and the Role of the Vaginal Microbiome
Published in Social Sciences, Cancer, and Microbiology
Figure 1. The Vaginal Microbiome, HPV infection, and Cervical Carcinogenesis: A Systematic Review in the Latina Population. Background, Approach, and Key Findings. Created on Canva, by Vianney Mancilla.
How it all began
The impetus for the systematic review began with findings from a cervical cancer cohort led by the Herbst-Kralovetz Lab in Phoenix, Arizona, in 2018 [1]. In a cohort of Arizonan women with equal participation of Hispanic and Non-Hispanic White women, the group identified associations between specific vaginal microbes, Hispanic ethnicity, and cervical carcinogenesis
During the summer of 2022, the Herbst-Kralovetz Lab welcomed a Blaiser/Frontera summer mentee, Vianney Mancilla. The University of Arizona’s Blaiser/Frontera program is designed to prepare undergraduate students historically underrepresented in medicine with the skills and experiences necessary to be competitive medical school applicants. Among these opportunities includes conducting a research project with a renowned faculty mentor at the University of Arizona. What began as an undergraduate summer literature search project that encapsulated Vianney’s passion for health disparities and women’s health and the Herbst-Kralovetz Lab’s interest in investigating cancer risk in Latina study populations, evolved into a systematic review on the topic of her summer research project.
The depletion of health-associated lactobacilli and overgrowth of anaerobes such as Gardnerella, Prevotella, Sneathia, Fannyhessea, and others are associated with bacterial vaginosis (BV). Bacterial vaginosis is linked to numerous adverse gynecologic sequelae and reproductive health outcomes. Notably, women with BV have an increased risk of acquiring sexually transmitted infections, including human papillomavirus (HPV) [2]. Over 90% of HPV infections are cleared, but persistent re-infection of high-risk HPV genotypes can lead to the premalignant precursor, cervical intraepithelial neoplasia (CIN), and ultimately, cervical cancer [3]. Evidence supports the dual role of the cervicovaginal microbiota in HPV clearance through lactobacilli dominance and HPV persistence and cervical cancer development promoted by a dysbiotic shift to BV-associated bacteria, respectively [4].
Latinas have among the highest rates of BV and HPV infection and are 40% more likely to be diagnosed with cervical cancer compared to other racial and ethnic groups [5,6]. This disparity could be exacerbated by systemic barriers that prevent Latinas from receiving access to adequate healthcare services, including HPV vaccination, cervical cancer screenings, and health education [7]. Despite experiencing the highest cervical cancer morbidity and mortality rates, the Latina population continues to be under researched in terms of the vaginal microbiome, HPV, and cervical cancer studies [6]. To identify key vaginal microbes from women more globally, we conducted a systematic review to identify bacteria reported in the VMB of Latinas relating to HPV infection, cervical dysplasia, and cervical cancer, as well as to better understand the role of the microbiome in these disease states across Latin America.
We gathered a team of diverse, inclusive, and transdisciplinary authors dedicated to paving a path to health equity. Our expertise in different areas including pathology, microbiology, epidemiology, and library and information science, allowed for integral conversations on structural racism and factors relating to health disparities while maintaining discussion on race and ethnicity as a non-biological factor. In addition, a number of the investigative team members also identify as Latina or Mexican-American (Chicana), offering a personal and unique perspective on this topic.
What we learned
PubMed, EMBASE, and Scopus databases were searched to include observational studies reporting on the cervicovaginal microbiota in premenopausal Latina women with HPV infection, cervical dysplasia, and cervical cancer. Twenty-five articles were eligible for final inclusion (N = 131,183), and forty-two unique bacteria were reported in the VMB of Latinas across ten countries in North, Central, and South America, and the Caribbean.
In multiple included studies, seven bacteria were consistently enriched across various stages of cervical carcinogenesis in Latinas. Lactobacillus crispatus was associated with health and dysplasia, whereas Lactobacillus iners was associated with health, HPV infection, and dysplasia. Three bacteria, Chlamydia trachomatis, Prevotella amnii, and Prevotella spp. were enriched in the HPV and dysplasia groups. Enrichment of Fusobacterium spp. was detected in Latinas with cervical cancer. Notably, emerging pathogens, Sneathia spp. were enriched across all stages of cervical carcinogenesis and continue to be microbes of interest in this setting.
Additional findings reported an enrichment of L. iners in Latinas, and increased rates of Lactobacillus depletion in Latinas compared to non-Latinas. Our study supports previously reported racial-ethnic differences in VMB composition which reveals an abundance of diverse anaerobes and a depletion of health-associated Lactobacillus species in Black and Hispanic women compared to White and Asian women [8, 9].
Why does this matter?
This study identifies 42 unique bacteria and consistent enrichment of seven bacteria across various stages of cervicovaginal carcinogenesis in Latinas. Longitudinal microbiome studies and larger cohort studies, including Latinas will help determine the role of these bacteria as drivers (influential disease-causing agents), passengers (less influential agents favoring the environment), or a consequence of disease in this population of women [10]. Additionally, varying study designs and a general lack of homogeneity of reported analyses (e.g., few and dissimilar statistical tests, differing variables, etc.) did not meet the basic criteria for unbiased meta-analytic methods. A call for standardization in study methods and analyses across VMB studies can lead to the development of robust conclusions on risk for HPV infection, cervical dysplasia, and cancer outcomes in Latinas. Lastly, advanced public health efforts, including community-based participatory research projects with Latinas, can reduce health disparities in HPV infection and cervical cancer [11].
Overall, this study provides insight to guide future cervicovaginal microbiome research to better inform cervical cancer prevention strategies in Latinas. Intervention through VMB modulation with lactobacilli-based probiotics may improve patient outcomes in this highly susceptible population by promoting HPV clearance and regression of disease [12].
To read more about our systematic review, read the full article in Journal of Epidemiology and Global Health: The Vaginal Microbiota, Human Papillomavirus Infection, and Cervical Carcinogenesis: A Systematic Review in the Latina Population
References
- Łaniewski P, Barnes D, Goulder A, et al. Linking cervicovaginal immune signatures, HPV and microbiota composition in cervical carcinogenesis in non-Hispanic and Hispanic women. Sci Rep. 2018;8(1):7593. Published 2018 May 15. doi:10.1038/s41598-018-25879-7
- Łaniewski P, Herbst-Kralovetz MM. Bacterial vaginosis and health-associated bacteria modulate the immunometabolic landscape in 3D model of human cervix. NPJ Biofilms Microbiomes. 2021;7(1):88. Published 2021 Dec 13. doi:10.1038/s41522-021-00259-8
- Plummer M, Schiffman M, Castle PE, Maucort-Boulch D, Wheeler CM, ALTS Group. A 2-year prospective study of human papillomavirus persistence among women with a cytological diagnosis of atypical squamous cells of undetermined significance or low-grade squamous intraepithelial lesion. J Infect Dis. 2007;195(11):1582–9. https://doi.org/10.1086/516784.
- Ntuli L, Mtshali A, Mzobe G, Liebenberg LJ, Ngcapu S. Role of Immunity and Vaginal Microbiome in Clearance and Persistence of Human Papillomavirus Infection. Front Cell Infect Microbiol. 2022;12:927131. Published 2022 Jul 7. doi:10.3389/fcimb.2022.927131
- Peebles K, Velloza J, Balkus JE, McClelland RS, Barnabas RV. High global burden and costs of bacterial vaginosis: a systematic review and meta-analysis. Sex Transm Dis. 2019;46(5):304–11. https://doi.org/10.1097/OLQ.0000000000000972.
- Ortiz AP, Soto-Salgado M, Calo WA, et al. Elimination of cervical cancer in U.S. Hispanic populations: Puerto Rico as a case study. Prevent Med. 2021;144:106336. https://doi.org/10.1016/j.ypmed.2020.106336.
- Almonte M, Albero G, Molano M, Carcamo C, García PJ, Pérez G. Risk factors for human papillomavirus exposure and co-factors for cervical cancer in Latin America and the Caribbean. Vaccine. 2008;26:L16–36. https://doi.org/10.1016/j.vaccine.2008.06.008.
- Ravel J, Gajer P, Abdo Z, et al. Vaginal microbiome of reproductive-age women. Proc Natl Acad Sci. 2011;108(supplement_1):4680–7. https://doi.org/10.1073/pnas.1002611107.
- MacIntyre DA, Chandiramani M, Lee YS, et al. The vaginal microbiome during pregnancy and the postpartum period in a European population. Sci Rep. 2015;5(1):8988. https://doi.org/10.1038/srep08988.
- Łaniewski P, Ilhan ZE, Herbst-Kralovetz MM. The microbiome and gynaecological cancer development, prevention and therapy. Nat Rev Urol. 2020;17(4):232. https://doi.org/10.1038/s41585-020-0286-z.
- Barnack-Tavlaris JL, Garcini L, Sanchez O, Hernandez I, Navarro AM. Focus group discussions in community-based participatory research to inform the development of a human papillomavirus (HPV) Educational Intervention for Latinas in San Diego. J Cancer Educ. 2013. https://doi.org/10.1007/s13187-013-0516-0517.
- Mei Z, Li D. The role of probiotics in vaginal health. Front Cell Infect Microbiol. 2022;12:963868. Published 2022 Jul 28. doi:10.3389/fcimb.2022.963868
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Public Health Professionals’ Education
Strengthening the education and continuous professional development of public health professionals is fundamental to achieving resilient, equitable, and responsive health systems. Recent global experiences including pandemics, protracted humanitarian crises, climate-related health risks, rapid urbanization, and technological transformation have highlighted both the critical role of the public health workforce and persistent gaps in training relevance, scale, quality, and sustainability. In many settings, educational models remain insufficiently aligned with real-world system needs, emerging competencies, and evolving career pathways.
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Artificial intelligence in health
How AI shapes medicine and human health
Artificial intelligence (AI) in health now spans large language models, computer vision, multimodal foundation models, predictive analytics, and decision-support systems. 1 2 These tools can process clinical text, imaging, physiological signals, laboratory data, patient history, and public health information at scale, and they are being piloted to support tasks ranging from documentation and prescription renewal to diagnostic interpretation, risk stratification, surveillance, and health-system operations.3 As adoption accelerates, evidence on real-world performance, safety, equity, and downstream health effects remains limited.
Large language models are one important branch of AI in health, but they are only part of a broader landscape that also includes computer vision for medical imaging, machine learning for prediction and classification, multimodal models that combine text and images or signals, and AI tools embedded in clinical and public health workflows. Across all of these approaches, performance depends on data quality, representativeness, validation, and the context in which the system is deployed.
LLMs are systems designed to predict the most likely next word. The word “Large” implies the vastness of written data accessible electronically—including books, web data, and articles—used in these predictions. The word “model” implies that LLMs do not know facts as humans do; they operate on probability. When we type or ask a question (called a prompt), the model predicts the next most likely option, called a “token,” based on the patterns it learned during training.1 This happens in a non-linear manner, meaning it processes words in relation to all other words in a sentence rather than one by one, helping it understand context.
In simple terms, the computing framework used in an LLM, the transformer, finds all possible routes on a map (neural networks) to reach a destination and then highlights the best options based on the logic it was trained to follow. The models are only as good as the data available to them and the training and fine-tuning they were subjected to.
For example, in Utah’s Prescription-Renewal Pilot Program3 launched in January 2026, LLMs are trained (the first 250 outputs require physician review for fine-tuning) and approved to perform an agentic function for 192 commonly prescribed chronic disease medicines that require frequent refills (a repetitive activity for physicians). Tethered to a deterministic logic engine, the system uses a multi-agent AI platform to handle the natural language interaction with the patient and data (patient records, patient-provided information on health status during the refill process, drug pharmacology, and common patient challenges).
AI can assist in patient care, public health surveillance, training of health care professionals, data monitoring, and policy development.2 4 5 Collective use of data can shape policy decisions in public health, including through rapid and live analysis of social media chats or disease report patterns to generate new hypotheses or public health responses.
Deployment of LLMs in healthcare is outpacing the evidence base for real-world performance, equity, and downstream population-health effects.
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As it is new and rapidly evolving, there is a need to surface critical concerns and risks about AI in health.
Mainstreaming AI is a challenge across disciplines, geographies, and development barriers. Most existing literature treats clinical AI and population-health AI as separate conversations. In practice, a tool deployed at the bedside has immediate epidemiologic and policy consequences—who gets referred, who gets screened, which signals reach surveillance systems, and which populations bear the costs of miscalibration. Public health is yet to exploit the vast analytic power of AI in triaging huge amounts of healthcare delivery data and health-related social media discussions, or the utility of AI in healthcare delivery, including supply chains. There is an imminent need to avert a potential AI access or utilization gap between resource-rich and resource-poor countries.
There is a policy vacuum and regulatory gap regarding AI applications and licensing.
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The challenge to researchers is to disseminate information on the potential utility and impact on the healthcare delivery system, and how to steer policy proactively to avert potential drawbacks that are common to all new approaches.
This collection is designed to connect those conversations and to produce evidence useful to clinicians, epidemiologists, and policymakers simultaneously, with particular attention to equity, low- and middle-income country (LMIC) applicability, and unintended consequences. We welcome original research, systematic reviews, policy analyses, and commentaries on generative and non-generative AI across the following areas:
- Clinical and diagnostic domains — development, validation, and real-world evaluation of diagnostic, prognostic, and decision-support tools; LLMs in clinical workflows; computer vision in radiology, pathology, dermatology, ophthalmology, endoscopy, and surgery; multimodal models integrating text, imaging, laboratory, and waveform data; clinician–AI interaction and human oversight.
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- Medical and health system research — development of prompts for large data use and highlighting utility to inform policy; innovations in surveillance systems; improving efficiency of scientific investigations, including methodology and survey development.
We believe the Collection will serve as inaugural contributions on this rapidly evolving interface of medicine, public health, biotechnology, and ethics, collectively enhancing our efforts to continuously improve human health and welfare.
References
1. Teo ZL, Thirunavukarasu AJ, Elangovan K, et al. Generative artificial intelligence in medicine. Nature Medicine 2025;31(10):3270-82. doi: 10.1038/s41591-025-03983-2
2. Rajpurkar P, Chen E, Banerjee O, et al. AI in health and medicine. Nature Medicine 2022;28(1):31-38. doi: 10.1038/s41591-021-01614-0
3. Gerke S, Parikh RB, Cohen IG. Utah’s Prescription-Renewal Pilot Program — Autonomous AI Managing Patient Care. New England Journal of Medicine 2026;394(16):1561-63. doi: doi:10.1056/NEJMp2601148
4. Panteli D, Adib K, Buttigieg S, et al. Artificial intelligence in public health: promises, challenges, and an agenda for policy makers and public health institutions. The Lancet Public Health 2025;10(5):e428-e32.
5. Rao VM, Hla M, Moor M, et al. Multimodal generative AI for medical image interpretation. Nature 2025;639(8056):888-96.
All submissions in this collection undergo the journal’s standard peer review process. Similarly, all manuscripts authored by a Guest Editor(s) will be handled by the Editor-in-Chief. As an open access publication, this journal levies an article processing fee (details here). We recognize that many key stakeholders may not have access to such resources and are committed to supporting participation in this issue wherever resources are a barrier. For more information about what support may be available, please visit OA funding and support, or email OAfundingpolicy@springernature.com or the Editor-in-Chief.
Publishing Model: Open Access
Deadline: Feb 26, 2027
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