Using Fiji as a simplified tool for assessing alveolar chord length and septal thickness in neonatal pulmonary disease research

This is the only paper in the entire literature of alveolar morphometry which describes the difference between chord length and mean linear intercept, and details a step-by-step method to download and install our customized plugin for measuring chord length on lung sections of different animal model

Published in Biomedical Research

Go to the profile of Pragnya Das
Pragnya Das
Scientist, Cooper University Hospital
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The idea was conceived in 2017-18 and finally got published in 2026. It all started with our initial ImageJ program getting corrupt and finding an alternative method to measure chord length on our lung sections. This will be a landmark paper (for me) in the sense that it involved 3 continents and the original chord length script creator (RH), in the entire process:

 Conceived in North America (PD), developed in Africa and Europe (EM).

 The longest ever time to get it published (from its first communication in July 2025 to its final publication in March 2026 ….. 8 months)

 Many journals rejected it and asked to limit it to 1200 words, but I wanted to include the historical aspect of how ImageJ was developed because we wanted other researchers to be aware of the founders of ImageJ, at least.

 Only paper (so far) to differentiate between Chord Length and Mean Linear Intercept and measure Septal Thickness on lung sections using our customized plugin

Go to the profile of Pragnya Das
Pragnya Das
Scientist, Cooper University Hospital

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