Using phosphatase inhibitor beads coupled with mass spectrometry (PIB-MS) to parse phosphoprotein phosphatase signaling.
In our Nature Protocol, we present step by step instructions on how to make phosphatase inhibitor beads, enrich lysates for phosphoprotein phosphatases, multiplex, phosphopeptide enrich, and analyze samples by mass spectrometry to systematically study the major drivers of protein dephosphorylation.
