Rossella Crescitelli is researcher at Sahlgrenska Center for Cancer Research, Department of Surgery in Roger Olofsson Bagge´s group. She has ten years of experience in the field of extracellular vesicles. Her research work is mainly focused on optimization of extracellular vesicle isolation protocol from body fluids and tissues. She has long experience in analysis of extracellular vesicles and tissues by transmission electron microscopy. After completing her Degree in Medical Biotechnologies (2005 – 2010) at University of Naples “Federico II, she moved in North of Italy for Doctoral studies in Biotechnologies for Human Health, University of Eastern Piedmont “Amedeo Avogadro” (2010-2014). During Doctoral studies, she has been invited by Prof. Jan Lötvall to work at Krefting Research Centre, University of Gothenburg (2011-2013). She worked as post doc fellow at Krefting Research Centre (2014-2019). From 2020 she is employed as researcher at Sahlgrenska Center for Cancer Research in Roger Olofsson Bagge´s group. She is expert in vesicles isolation and characterization as well as she acquired experience in electron microscopy demonstrated by scientific publications in high-quality journals like Nature Protocols, Leukemia, Gene and Journal of Extracellular Vesicles. She established a new protocol to distinguish different subpopulations of extracellular vesicles isolated from cell line supernatants. The results were published on Journal of Extracellular Vesicles and the paper was the most cited in 2014 among the articles published on that journal. She recently optimized a protocol to isolate subpopulations of extracellular vesicles from tumor tissues. The protocols were published in Nature Protocols in January 2021. She is currently working on isolation and characterization of vesicles from lymphatic exudates from breast cancer patients and analysis of DNA in extracellular vesicle subpopulations isolated from melanoma metastases.