Evaluation of Anticholinesterase Activity of Orthoclase and Kaolin from Selected Yemeni Stones: Potential Anti-Alzheimer Agents

DRUG DISCOVERY FROM NATURAL SOURCE

Published in Chemistry and Biomedical Research

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Antibacterial and antioxidant properties of selected Yemeni stones: potential for safe and cost-effective therapeutic applications - Discover Applied Sciences

Background Yemen’s diverse geological environment offers a variety of stone types that may possess significant medical benefits. In particular, some Yemeni stones have demonstrated antimicrobial and antioxidant activities, suggesting their potential for use in developing cost-effective antibacterial agents. Objective This study aimed to evaluate the antimicrobial and antioxidant properties of selected Yemeni stone powders and assess their potential as effective, and inexpensive antibacterial agents. Methodology Stone samples were collected from different regions of Yemen between September 2022 and November 2022. The antimicrobial activity was assessed using well diffusion assays, followed by determination of the minimum inhibitory concentration (MIC) and minimum bactericidal/fungicidal concentration (MBC/MFC) via serial dilution techniques. Two extraction solvents (DMSO and ethanol) were used, and results were expressed as mean ± SEM. Antioxidant activity was evaluated using the DPPH radical scavenging assay, with vitamin C serving as the positive control. Results The Yemeni stone powders exhibited variable biological activity. For extracts dissolved in DMSO, Tuff (Sample 1) showed strong broad-spectrum antimicrobial activity with MIC values ranging from 0.16 ± 0.00 mg/mL for Pseudomonas aeruginosa to 0.47 ± 0.03 mg/mL for Escherichia coli and MRSA, while Kaolin (Sample 13) demonstrated the strongest antimicrobial effect with an MIC of 0.424 mg/mL and an MBC of 0.683 mg/mL. In antioxidant assays, Orthoclase (Sample 2) exhibited the most potent free radical scavenging ability with an IC₅₀ of 23.08 ± 0.78 mg/100 µL, approaching the efficacy of vitamin C (IC₅₀ = 13.93 ± 1.12 mg/100 µL). Other stone samples, including Hematite, Limestone, Quartz, Marble, and Fluorite, displayed moderate activity, with MIC values between 0.1563 mg/mL and 0.683 mg/mL and antioxidant IC₅₀ values ranging from 36.03 ± 1.44 mg/100 µL to 80.29 ± 0.79 mg/100 µL. Conclusions The findings indicate that certain Yemeni stone powders, particularly Kaolin (Sample 13) and Orthoclase (Sample 2), possess promising antimicrobial and antioxidant properties. The strong antibacterial activity (e.g., an MIC of 0.424 mg/mL for Kaolin) and potent antioxidant activity (IC₅₀ of 23.08 ± 0.78 mg/100 µL for Orthoclase) suggest that these stones could serve as natural, cost-effective sources for developing new antibacterial agents.

Background and Objectives

There are many Yemeni stones that have demonstrate different biological activities that could be used as source of promising drugs. These stones can have interesting activities .the stones Are effectiveness as antimicrobial ,antioxidant and also anti-Alzheimer's diseases .Therefore, this study will concern on the study of some selected stones as antimicrobial and anti-oxidant activities and evaluate the in vivo activity of stones as anti-Alzheimer activity. To elevate the targeted biological activities ,stones were collected based on the present of silica , aluminum and other minerals for activities .

Materials and Methods

 The collected stones responsible. Were studied between the period of collected stones were between December 2023 to October 2023. The stones were collected from different places in Yemen .Then the anti-microbial activity Escherichia coli , Klebsiella Staphylococcus aurous , Enterobacter , Candida were evaluated by measuring the inhibition zone and MIC(minimum inhibition zone ).The antioxidant activity were measured by determining the free radical scarving activity by DPPH. While the in- vivo activity of Orthoclase and Kaolin as anti-Alzheimer activity was elevated by measuring the behavior activity of the water maze test. Followed by conducting histopathology to the tested rats

Results

The Yemeni stones show significant biological activity, and antimicrobial activity against many types of G+ and G- bacteria and candida, the stones show stronger activity against G-+ve bacteria rather than G-ve bacteria with MIC. The collected kaolin sample showed the strongest antimicrobial activity with MIC 0.424 mg and MBC 0.683mg.Evaluation of the samples as antioxidant were strong, the orthoclase showed the strongest activity. In Alzheimer's disease the orthoclase sample showed the strongest activity when compared with the standard drug Donepezil with IC50 0.081ꭒg/ml the Orthoclase and Kaolin compared with donepezil were showed promosing activity and improvement the behavioral activity at the Morris test. In while Orthoclase and Kaolin give escape latency time 15,77 second compared with standard  drug donepezil 14,9 second and non-treated mice 57,44 and normal rat 14,89 seconds

Conclusion

The results of the current study confirm the antimicrobial, antioxidant, and anti-Alzheimer disease of some Yemeni stones. The stone kaolin which was collected from Amran, Saada shows antimicrobial activity. the stone orthoclase shows good anti-oxidant activity and anti-Alzheimer disease. All biological activity achieved by stones might be associated with the presence of silicon and calcium in high concentrations

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Biomedical Research
Life Sciences > Health Sciences > Biomedical Research
Drug Delivery
Physical Sciences > Chemistry > Inorganic Chemistry > Organometallic Chemistry > Medicinal Chemistry > Drug Delivery

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