Behind the Paper
The real stories behind the latest research papers, from conception to publication, the highs and the lows
Filtered by: Protocols & Methods
Circular Vectors better than plasmids for RNA guide delivery and expression in cell culture experiments
We have developed an efficient, fully synthetic method and protocol called the Circular Vector method, which enables the production of circularized DNA containing expression elements that are ready for transfection in as little as 3 hours, thereby eliminating all bacterial cloning steps.
Ultraflat graphene membrane shapes uniform thin ice for cryo-EM analysis
Ultraflat graphene membrane is developed here as the supporting film for cryo-EM specimen preparation, which improves the image quality of vitrified samples and enables the high-resolution reconstruction of small macromolecules (<100KD).
