Behind the Paper
The real stories behind the latest research papers, from conception to publication, the highs and the lows
From a duo to a quartet: viral and bacterial symbionts influence fungal biology
By Laila P. Partida-Martínez
KAS-seq: capturing global transcription activity with kethoxal-assisted labeling
We describe a kethoxal-assisted single-stranded DNA sequencing (KAS-seq) approach. KAS-seq allows rapid (within 5 min), sensitive and genome-wide capture and mapping of ssDNA produced by transcriptionally active RNA polymerases or other processes in situ using as few as 1,000 cells.
Dysbiosis discovered in plants
Most of us are familiar with the term “Inflammatory Bowel Disease (IBD)”. However, you likely have not heard about “Inflammatory Phyllosphere Disease (IPD)”. This is because I have just made it up to draw your attention, and to describe a form of dysbiosis in plants that we have just reported.
Spatial Heterogeneity in Molecular Brightness
Oligomerisation of membrane proteins remains a field characterized by intense research interest, due to the large number of pharmacological and clinical implications that the formation of molecular complexes of signaling proteins carries.
Do fishes really get smaller with warming?
Experimental evidence shows that ectotherms grow to smaller sizes in warmer temperatures. Yet, it is not clear how this affects average body sizes in wild populations, where growth, mortality, recruitment and interactions with other organisms all occur simultaneously.
