Ethanol pretreatment stimulates molecular processes that help crops survive heat stress during summer

Published in Sustainability
Ethanol pretreatment stimulates molecular processes that help crops survive heat stress during summer
Like

Share this post

Choose a social network to share with, or copy the shortened URL to share elsewhere

This is a representation of how your post may appear on social media. The actual post will vary between social networks

Read the paper

SpringerLink
SpringerLink SpringerLink

Ethanol induces heat tolerance in plants by stimulating unfolded protein response - Plant Molecular Biology

Key message Ethanol priming induces heat stress tolerance by the stimulation of unfolded protein response. Abstract Global warming increases the risk of heat stress-related yield losses in agricultural crops. Chemical priming, using safe agents, that can flexibly activate adaptive regulatory responses to adverse conditions, is a complementary approach to genetic improvement for stress adaptation. In the present study, we demonstrated that pretreatment of Arabidopsis with a low concentration of ethanol enhances heat tolerance without suppressing plant growth. We also demonstrated that ethanol pretreatment improved leaf growth in lettuce (Lactuca sativa L.) plants grown in the field conditions under high temperatures. Transcriptome analysis revealed a set of genes that were up-regulated in ethanol-pretreated plants, relative to water-pretreated controls. Binding Protein 3 (BIP3), an endoplasmic reticulum (ER)-stress marker chaperone gene, was among the identified up-regulated genes. The expression levels of BIP3 were confirmed by RT-qPCR. Root-uptake of ethanol was metabolized to organic acids, nucleic acids, amines and other molecules, followed by an increase in putrescine content, which substantially promoted unfolded protein response (UPR) signaling and high-temperature acclimation. We also showed that inhibition of polyamine production and UPR signaling negated the heat stress tolerance induced by ethanol pretreatment. These findings collectively indicate that ethanol priming activates UPR signaling via putrescine accumulation, leading to enhanced heat stress tolerance. The information gained from this study will be useful for establishing ethanol-mediated chemical priming strategies that can be used to help maintain crop production under heat stress conditions.

Global warming, which has resulted in increased yield losses among agricultural crops, is a serious problem worldwide that must be solved to establish sustainable societies. Thus, agricultural sustainability depends on the development of technologies that can minimize crop yield losses.

Genetic engineering is a useful technique for enhancing stress tolerance; however, there is a demand for affordable, simple, and environmentally friendly technology that can increase crop stress tolerance, but does not require genetic engineering. This is because genetically modified plants are not readily available in all countries. Accordingly, pretreating crops with safe chemical compounds is a promising approach. Scientists have assessed the viability of this approach by analyzing various chemicals in terms of whether they can increase plant tolerance to environmental stresses.

In this study, we demonstrated that a pretreatment with ethanol at a low concentration  (i.e., 0.12%, which corresponds to the alcohol content of 100-fold diluted wine) increases heat tolerance without suppressing plant growth (Fig. 1). Hence, this pretreatment represents a cost effective, simple, and environmentally friendly way to protect crops from the detrimental effects of global warming (Fig. 1).

                          

We grew Arabidopsis, a non-crop plant often used for research, as well as lettuce and then added a low concentration of ethanol to the soil for several days. The ethanol-treated and untreated (control) 3-week-old Arabidopsis plants were then grown for 3 hr at temperature (50 ℃) high enough to induce heat stress. Only 10% of the control plants survived, whereas up to 70% of the ethanol-treated plants survived, reflecting the beneficial effects of the ethanol pretreatment (Fig. 2A). The ethanol pretreatment also improved the leaf growth of heat-stressed lettuce plants under field conditions (Fig. 2B).

We analyzed the molecular mechanisms underlying the effect of the ethanol pretreatment and identified a set of genes as well as a biochemical process activated by ethanol. Specifically, we detected the stimulation of the stress response known as the unfolded protein response (UPR) involving bZIP60 and Binding Protein 3 (BIP3) (Fig. 3), which function in an organelle called the endoplasmic reticulum (ER) to mitigate the effects of misfolded proteins that are produced during an exposure to environmental stress.

We also revealed that the ethanol pretreatment enhances the tolerance of various plants to drought (https://doi.org/10.1093/pcp/pcac114; https://link.springer.com/article/10.1007/s11103-022-01300-w) and high salinity (https://www.frontiersin.org/articles/10.3389/fpls.2017.01001/full), including rice, wheat, cassava, and Arabidopsis. We will further characterize the associated molecular mechanism and clarify the ethanol-mediated network enabling plants to adapt to environmental stress. Elucidating the mechanism may lead to the development of methods that improve and fine-tune the protective effect in a broad range of plant species.

Ethanol is cheaper and more readily available than other chemicals and reagents. Moreover, it is widely used as a disinfectant and food additive. Therefore, the ethanol pretreatment of crops may be acceptable to the general public. We intend to promote the use of this pretreatment as an agronomic practice that mitigates the adverse effects of global warming on crop production.

 

Please sign in or register for FREE

If you are a registered user on Research Communities by Springer Nature, please sign in