Each month, the Nature Methods editors get together to survey and discuss the methods literature, so that we can select candidates for the Research Highlights and In Brief section. Unfortunately, we can't highlight all the papers we are excited about. Here is a selection of additional recent papers that we thought might interest our readers.

Jaganathan, K. et al. Predicted splicing from primary sequence with deep learning

A deep learning-based tool, SpliceAI, predicts splice junctions from pre-mRNA transcript sequences.

Zhang, R.K. et al. Enzymatic assembly of carbon-carbon bonds via iron-catalysed sp3 C-H functionalization

Engineered, iron-based catalytic proteins functionalize diverse substrates through a C-H alkylation mechanism.

Hauri, S. et al. Rapid determination of quaternary protein structures in complex biological samples

Protein complex structures are determined using crosslinking-mass spectrometry and protein structure modeling.

Spahn, C. et al. Whole-Cell, 3D, and Multicolor STED Imaging with Exchangeable Fluorophores

3D STED with exchangeable fluorophores overcomes photobleaching-related limitations of the imaging technology.

Oliinyk, O.S. et al. Smallest near-infrared fluorescent protein evolved from cyanobacteriochrome as versatile tag for spectral multiplexing

miRFP670nano is the smallest monomeric infrared fluorescent protein and can be used for internal tagging or as a FRET donor.

Albert, P.S. et al. Whole-chromosome paints in maize reveal rearrangements, nuclear domains, and chromosomal relationships

Whole-chromosome oligo-FISH probes for each of the 10 chromosomes of maize can uncover aberrations and visualize dynamic behaviors of the chromosomes.

Diederich, B. et al.  cellSTORM—Cost-effective super-resolution on a cellphone using dSTORM

Affordable super-resolution microscopy on a cell phone achieves 80-nm or better resolution.

Bando, Y. et al. Comparative Evaluation of Genetically Encoded Voltage Indicators

GEVIs are compared under 1-photon and 2-photon illumination in cell culture and in vivo.

Wu, L. et al. PARIS, an optogenetic method for functionally mapping gap junctions

A fully genetically encoded tool for visualizing gap-junction coupling is applied in cell culture and in Drosophila brains.

Giovannucci, A. CaImAn an open source tool for scalable calcium imaging data analysis

CaImAn provides open-source tools for pre-processing calcium imaging datasets and identifying neurons in these datasets.

Enjoy these reads!