About Qing Dai
Trained as a Ph.D. in organic chemistry, I have been working on nucleic acid chemistry and synthesized many DNA and RNA oligos containing various modifications as models to study biochemistry. Since 2012, a K01 award from NIH allowed me to extend my research to chemical biology, mainly focusing on developing new sequencing methods for various DNA and RNA modifications.
Popular Content
Functional studies of RNA pseudouridine (Ψ) modification have been limited by lacking quantitatively map individual Ψ sites transcriptome-wide. To address this challenge, Dai et al. developed BID-seq, a method for quantitative mapping of Ψ at single-nucleotide resolution.
UBS-seq: An ultrafast bisulfite sequencing method more accurately detecting 5-methylcytosine in DNA and RNA
Current bisulfite sequencing (BS-seq) suffers from notable limitations such as long reaction time, severe DNA damage, overestimation of modification level etc. UBS-seq overcomes these limitations and detects 5-methylcytosine in DNA and RNA accurately starting from low input biological samples.