Using clusterProfiler to characterise Multi-Omics Data

ClusterProfiler is an R package designed for enrichment analysis to identify perturbed pathways and uncover potential molecular mechanisms. This protocol outlines methods for using clusterProfiler to analyze various omics data, including metabolomics, metagenomics, and transcriptomics.
Using clusterProfiler to characterise Multi-Omics Data
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ClusterProfiler was developed over a decade ago and first published in OMICS in 2012.  It was created to address the emerging need for complex experiment designs involving multiple time points and conditions, as simple case-control setups were predominant at that time. The package has continuously evolved, featuring an exceptionally user-friendly interface. It has now become a popular tool in the bioinformatics community. 

ClusterProfiler offers several key features that make it stand out from similar tools. It facilitates online retrieval of the latest genome annotation, supporting Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis for thousands of species. Additionally, it provides a universal interface for incorporating user-provided custom annotation, enabling analysis of new species and utilization of new functional annotation. Notably, clusterProfiler allows for the utilization of genomic regions, enabling enrichment analysis of epigenomic data. Moreover, it implements comparative analysis of multiple datasets to support complex experimental designs and offers a tidy interface, streamlining data manipulation and interpretation. 

This protocol mainly introduces three user cases, demonstrating the functionality of comparing biological themes in disease subtypes, analyzing functions of perturbed transcription factors, and annotating cell types using various omics data sets, including metagenomics, metabolomics, transcriptomics, and single-cell omics. 

It is not limited to the types of omics data presented and can be used to uncover potential molecular mechanisms from the user's chosen perspective. 

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