Reversal of the flagellar motor using an optical torque
We fabricated a microstructure called a “light-mill” that rotates under optical trapping with a frequency (and torque) determined by the intensity of the trapping laser and we coupled it to the rotation of a single flagellar motor, in order to measure its torque-speed curve.
Published in Materials, Physics, and Protocols & Methods
Like
Be the first to like this
Check out our new publication!
An optically driven microstructure for torque measurement in rotary molecular motors

We fabricated a microstructure called a “light-mill” that rotates under optical trapping with a frequency (and torque) determined by the intensity of the trapping laser. We coupled the light-mill to the rotation of a flagellar motor and, by modifying the optical torque, we were able to measure the torque of the motor in both clockwise and counterclockwise directions.
Any feedback or discussion will be really appreciated!
Follow the Topic
Media Education
Humanities and Social Sciences > Education > Media Education
Molecular Biophysics
Physical Sciences > Physics and Astronomy > Biophysics > Molecular Biophysics
Optical Tweezers
Life Sciences > Biological Sciences > Biological Techniques > Biophysical Methods > Optical Tweezers
Motor Protein Structure
Physical Sciences > Physics and Astronomy > Biophysics > Motility > Motor Protein Structure
Molecular Machines and Motors
Physical Sciences > Materials Science > Nanotechnology > Nanoscale Devices > Molecular Machines and Motors
Cell Mechanics and Motility
Physical Sciences > Physics and Astronomy > Biophysics > Cell Mechanics and Motility
Please sign in or register for FREE
If you are a registered user on Research Communities by Springer Nature, please sign in