Behind the Paper
The real stories behind the latest research papers, from conception to publication, the highs and the lows
Filtered by: Protocols & Methods
Designing next-generation norepinephrine indicators for in vivo imaging
We engineered a novel family of genetically-encoded fluorescent norepinephrine indicators in green and red. These indicators exhibit exceptional sensitivity, ligand-specificity, and temporal resolution, surpassing prior indicators and enabling the detection of norepinephrine in living animals.
Increasing single-cell data completeness and depth of coverage with prioritized proteomics
Prioritized analysis enables proteins of interest to be consistently quantified across single-cell samples at no cost to depth of coverage. Applied to primary macrophages, prioritization facilitated the quantification of proteolytically regulated proteoforms and the study of endocytic competency.
Epichaperomics: Illuminating the Secrets of Protein Interactions in Disease Research
Introducing Epichaperomics: an emerging omics platform for research to illuminate the secrets of diseases. By studying protein interactions in cells and tissues, it offers valuable insights into disease mechanisms and paves the way for personalized therapies.
Unveiling the Role of Protein Glycosylation: Insights into Conformational Dynamics and Disease Mechanisms
Glycosylation plays a critical role in controlling normal cell functions as well as disease-related conditions. Understanding how sugar modifications impact proteins, including their conformations and interactions, is crucial for unraveling the causes of diseases and developing targeted treatments.
Finding the hidden needle: a workflow for 3D cryo-correlative light and electron microscopy volume imaging
Imagine capturing 3D images of whole tissue in an unperturbed hydrated state, transitioning from 3D cryo-light microscopy to 3D cryo-electron microscopy. Now this is possible with our 3D cryoCLEM workflow.
SproutAngio: An Open‑source Bioimage Informatics Tool for Quantitative Analysis of Blood Vessels
The new SproutAngio tool provides an automated and accurate tool for the quantitation of angiogenic sprouts and vessel structures from bioimages. SproutAngio is open-source, accessible, and approachable to all researchers without programming experience.
